Introduction: As one of the most commonly known skin issues, wounds remain a significant threat to public health and the world's economy despite the healing rates with advances in topical ointments, dressing, ultraviolet radiation, and hyperbaric oxygen therapy. Wound healing comprises four consecutive and overlapping stages. Interruption of any phase will lead to abnormal wound healing. The damaged skin will lead to the loss of skin integrity, resulting in an imbalance of functional balance, and even accompanied by disability or septic death. Irisin is an exercise-induced hormone; some research showed that irisin could perform neuroprotective, anti-inflammatory activities, anti-cancer activities, and attenuate pain.
Material and methods: CCD-966sk and HaCaT were seeded in culture-insert and treated with irisin (0.5 and 1 μg/mL), which was observed using microscopy at various time points. Four full-thickness wounds of 6 mm diameter were created on the
dorsal skin of each rat in all treatment groups using a biopsy skin punch. The wound closure rate was recorded and determined by using ImageJ software. The immunohistochemical staining were analyze the expression of the proteins.
Result: Our studies show that irisin not only enhanced wound healing with the potential mechanism of increasing the capability of migration in vitro but accelerated the wound closure rate and increased the collagen III, SNAP25, and TGF-β1 protein
expression in a dose-dependent manner, which is related to inflammation, stimulating angiogenesis, fibroblast proliferation, and collagen synthesis in vivo.
Conclusion: Irisin is a potential protein drug to enhance wound healing capability.

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