The Author, working in a close manner with the most renowned Masters of Body Contouring and Fat Transfer worldwide, realized the tremendous potential of Adipose Tissue in most of the surgical strategies.
Thanks to the tremendous contribution of many skilled Plastic Surgeons and also to a better understanding of the Adipose Tissue Patho-Physiology has been able to progressively lay the foundations of a new well identified surgical sub-specialty termed LIPOPLASTY.
For very long time Lipoplasty has been mainly focused on the volumetric approach of the Adipose Tissue body deposits both for volume reduction (Traditional Lipoaspiration, Power Assisted Lipoplasty, Ultrasonic Assisted Lipoplasty, Ultrasonic Megalipoplasty) and volume augmentation (Autologous Collagen, Lipostructure, Lipocondensation).
The lipo-transfer, commonly termed lipofilling, had a rapid diffusion among Plastic and Aesthetic Surgeons as the most reliable, affordable and efficient way to treat many volumetric defects, offering important advantages and benefits if compared to all the other ready-to-use products.
Many, if not all, patients treated with this technique show an improved quality of the skin and superficial layers both for elasticity and texture. This evidence clearly indicates that the transfer of fat tissue is acting not only as a volume replacement but also as a powerful tissues regenerator. This clinical evidence has been at the origin of the Author's increasing interest on AT regenerative potential stimulating the transition from Volume Replacement to Regeneration.
In the late 80's the Author developed and popularized a novel surgical technique for separating the different components of the Adipose Tissue and extracting the ECM for facial rejuvenation.
In 1989 he published the very first article ever published on adipose tissue fragmentation and centrifugation.[1]
In this technique, commonly known as Autologus Collagen, the condensed Adipose Tissue was emulsified for 60 seconds by sonication and than centrifugated for 2 min at 450 G. Unfortunately at that time, aiming to improve the rate of volume persistence, the Author totally focused his attention on the tissutal fraction only disregarding the infra-natant cellular fraction that was the richest one in regenerative components.
Only 12 years later (2001) P. Zuk described the ADCSc contained in the lower fraction, opening a new chapter in the history of Lipo-transfer. [2]

DIFFERENT TYPES OF STEM CELLS
Stem cells are categorized based on their potency or on the diversity of cell types they can become as they differentiate.
Totipotent stem cells can divide into all cell types in an organism. A totipotent cell has the potential to divide until it creates an entire, complete organism.