Abstract:
The Ambystoma mexicanum (aka axolotl) are highly regenerative and have provided numerous insights into the principles and mechanisms of tissue and organ regeneration. Upon injury, axolotls generate a specialized tissue known as “blastema”, which harbors a population of mesenchymal progenitors. Intriguingly, using glands as a definitive hallmark of skin regeneration, we observed prominent delay in skin regeneration in blastema-forming wounds. Here, we used the live imaging to depict the cellular events and the scRNAseq to identify specialized skin-fated fibroblasts guiding gland formation. Signaling regulators of gland regeneration in amphibians is conserved and thus the knowledge learned from the axolotl model can potentially be translated to better understand mechanisms in scarless healing and skin appendages regeneration in mammals.

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